Below are a few
major topics for this chapter.
Note: these are
NOT the only topics you should know;
you are expected to master everything discussed in this chapter.
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Hint
Reproductive cloning by somatic cell nuclear transfer
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Hint
Embryonic and adult stem cells: totipotent and pluripotent development
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Hint
Cleavage: production of a blastocyst with inner cell mass
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Hint
Therapeutic cloning: somatic cell nuclear transfer and stem cells
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Hint
Restriction enzyme: restriction sites, sticky ends, recombinant DNA
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Hint
PCR: denaturation, primer annealing, primer extension
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Hint
Gel electrophoresis: separation of DNA fragments
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Hint
DNA fingerprinting: identifying individuals by restriction fragments
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Hint
Genetic engineering applications: "pharm" organisms
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Hint
GM plants: pest and herbicide resistance
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Hint
Human genome: genes, introns, repeated sequences, structural DNA, transposable elements
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Hint
Genome sizes: variation among species